Research Assistant
Duke-NUS Medical School
College Road, Singapore
16d ago

Job Description

This position is intended to provide assistance with the development of a human cell therapy for heart injury. The main aim of the study is to generate clinical quality hESC-

derived cardiomyocyte progenitor cell for regenerative cardiology in pigs with myocardial infarction and subsequent first-

in-man clinical trial for cell therapy of heart disease. The selected candidate shall perform a variety of functions related to research under the supervision of the Principal Investigator or his / her designate : -

  • Culturing and differentiation of embryonic stem cells. Cell sorting using FACS and isolation of RNA and analyses using qPCR and RNAseq.
  • Culturing and differentiating hES cells to cardiomyocytes and progenitors. This includes plating and culturing of the cells, freezing cells for future storage, collection of cell culture media and being responsible for that all reagents and supplies for this part project are in order.
  • Protein purification with liquid chromatography, protein analyses using SDS-PAGE and western blotting.
  • Preparation of tissue samples for histological, histochemical and immunohistological analyses, using microscopy and immunological imaging.
  • Preparation of cell culture media, coating of culture plates and flasks with recombinant proteins.
  • Collaborate with animal surgeons in mouse and pig surgery. Collaborate with human cardiac surgeons and prepare high quality hES cell derived cardiomyocyte progenitors and assist with injection of the cells into the hearts by injections or catheters.
  • Collaborate with the Laboratory Manager and other Research Associates as well as investigators of the group to ensure proper management and order of the laboratory.
  • Preparation and maintenance of laboratory notebooks, evaluation and interpretation of collected data and preparation of research reports.
  • Job Requirements

  • Bachelor Degree in biology / molecular biology, MSc degree is to be preferred.
  • Experience with cell biology, including stem cell research and experimental animal handling. Other essential experience includes molecular biology, protein analyses, genetic engineering and bioreactor system.
  • Theoretical knowledge in biology / developmental biology, biochemistry and molecular biology.
  • Familiar with sterile surgery operation practice.
  • Laboratory experimental skills.
  • Strong team player skills and the ability to work harmoniously with a diverse workforce.
  • Self-motivation, good oral and written communications skills.
  • We regret that only shortlisted candidates will be notified.

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